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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
03/05/2021 |
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Data da última atualização: |
03/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MATHIOUDAKIS, M. M.; MALIOGKA, V. I.; CANDRESSE, T.; NICKEL, O.; FAJARDO, T. V. M.; BUDZYNSKA, D.; JAROSZEWSKA, B. H.; KATIS, N. I. |
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Afiliação: |
MATTHAIOS M. MATHIOUDAKIS, Plant Pathology Laboratory, Institute of Olive Tree, Subtropical Plants & Viticulture, ELGO-DIMITRA, Karamanlis Ave. 167, Gr-73134 Chania, Greece; VARVARA I. MALIOGKA, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, Gr-54124 Thessaloniki, Greece; vmaliogk@agro.auth.gr (V.I.M.); katis@agro.auth.gr (N.I.K.); THIERRY CANDRESSE, INRAE, UMR BFP, University of Bordeaux, CS20032, 33882 Villenave d’Ornon CEDEX, France; thierry.candresse@inrae.fr; OSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV; DARIA BUDZYNSKA, Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, ul. W?. W?egorka 20, 60-318 Pozna ´n, Poland; d.budzynska@iorpib.poznan.pl (D.B.); b.hasiow@iorpib.poznan.pl (B.H.-J.) * Correspondence: mathioudakis@elgo.; BEATA HASIOW JAROSZEWSKA, Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, ul.; NIKILAOS I. KATIS, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, Gr-54124 Thessaloniki, Greece. |
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Título: |
Molecular characterization of the coat protein gene of greek apple stem pitting virus isolates: evolution through deletions, insertions, and recombination events. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plants, v. 10, p. 1-19, e10050917, abril 2021. |
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Páginas: |
19 |
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DOI: |
10.3390/plants10050917 |
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Idioma: |
Inglês |
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Conteúdo: |
A RT-PCR assay developed to amplify the full coat protein (CP) gene of apple stem pitting virus (ASPV) was evaluated using 180 Greek apple and pear samples and showed a broad detection range. This method was used to investigate the presence of ASPV in quince in Greece and showed a high incidence of 52%. The sequences of 14 isolates from various hosts with a distinct RFLP profile were determined. ASPV population genetics and the factors driving ASPV evolution were analyzed using the Greek ASPV sequences, novel sequences from Brazilian apple trees and Chinese botanical Pyrus species, and homologous sequences retrieved from GenBank. Fourteen variant types of Greek, Brazilian and botanical isolates, which differ in CP gene length and presence of indels, were identified. In addition, these analyses showed high intra- and inter-group variation among isolates from different countries and hosts, indicating the significant variability present in ASPV. Recombination events were detected in four isolates originating from Greek pear and quince and two from Brazilian apples. In a phylogenetic analysis, there was a tendency for isolates to cluster together based on CP gene length, the isolation host, and the detection method applied. Although there was no strict clustering based on geographical origin, most isolates from a given country tended to regroup in specific clusters. Interestingly, it was found that the phylogeny was correlated to the type, position, and pattern of indels, which represent hallmarks of specific lineages and indicate their possible role in virus diversification, rather than the CP size itself. Evidence of recombination between isolates from botanical and cultivated species and the clustering of isolates from botanical species and isolates from cultivated species suggest the existence of a possible undetermined transmission mechanism allowing the exchange of ASPV isolates between the cultivated and wild/ornamental hosts. MenosA RT-PCR assay developed to amplify the full coat protein (CP) gene of apple stem pitting virus (ASPV) was evaluated using 180 Greek apple and pear samples and showed a broad detection range. This method was used to investigate the presence of ASPV in quince in Greece and showed a high incidence of 52%. The sequences of 14 isolates from various hosts with a distinct RFLP profile were determined. ASPV population genetics and the factors driving ASPV evolution were analyzed using the Greek ASPV sequences, novel sequences from Brazilian apple trees and Chinese botanical Pyrus species, and homologous sequences retrieved from GenBank. Fourteen variant types of Greek, Brazilian and botanical isolates, which differ in CP gene length and presence of indels, were identified. In addition, these analyses showed high intra- and inter-group variation among isolates from different countries and hosts, indicating the significant variability present in ASPV. Recombination events were detected in four isolates originating from Greek pear and quince and two from Brazilian apples. In a phylogenetic analysis, there was a tendency for isolates to cluster together based on CP gene length, the isolation host, and the detection method applied. Although there was no strict clustering based on geographical origin, most isolates from a given country tended to regroup in specific clusters. Interestingly, it was found that the phylogeny was correlated to the type, position, and pattern of indels, which ... Mostrar Tudo |
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Palavras-Chave: |
ASPV. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/223000/1/plants-10-00917.pdf
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Marc: |
LEADER 02725naa a2200241 a 4500
001 2131631
005 2021-05-03
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3390/plants10050917$2DOI
100 1 $aMATHIOUDAKIS, M. M.
245 $aMolecular characterization of the coat protein gene of greek apple stem pitting virus isolates$bevolution through deletions, insertions, and recombination events.$h[electronic resource]
260 $c2021
300 $a19
520 $aA RT-PCR assay developed to amplify the full coat protein (CP) gene of apple stem pitting virus (ASPV) was evaluated using 180 Greek apple and pear samples and showed a broad detection range. This method was used to investigate the presence of ASPV in quince in Greece and showed a high incidence of 52%. The sequences of 14 isolates from various hosts with a distinct RFLP profile were determined. ASPV population genetics and the factors driving ASPV evolution were analyzed using the Greek ASPV sequences, novel sequences from Brazilian apple trees and Chinese botanical Pyrus species, and homologous sequences retrieved from GenBank. Fourteen variant types of Greek, Brazilian and botanical isolates, which differ in CP gene length and presence of indels, were identified. In addition, these analyses showed high intra- and inter-group variation among isolates from different countries and hosts, indicating the significant variability present in ASPV. Recombination events were detected in four isolates originating from Greek pear and quince and two from Brazilian apples. In a phylogenetic analysis, there was a tendency for isolates to cluster together based on CP gene length, the isolation host, and the detection method applied. Although there was no strict clustering based on geographical origin, most isolates from a given country tended to regroup in specific clusters. Interestingly, it was found that the phylogeny was correlated to the type, position, and pattern of indels, which represent hallmarks of specific lineages and indicate their possible role in virus diversification, rather than the CP size itself. Evidence of recombination between isolates from botanical and cultivated species and the clustering of isolates from botanical species and isolates from cultivated species suggest the existence of a possible undetermined transmission mechanism allowing the exchange of ASPV isolates between the cultivated and wild/ornamental hosts.
653 $aASPV
700 1 $aMALIOGKA, V. I.
700 1 $aCANDRESSE, T.
700 1 $aNICKEL, O.
700 1 $aFAJARDO, T. V. M.
700 1 $aBUDZYNSKA, D.
700 1 $aJAROSZEWSKA, B. H.
700 1 $aKATIS, N. I.
773 $tPlants$gv. 10, p. 1-19, e10050917, abril 2021.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
20/12/2012 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
SIQUEIRA, K. B.; PINHA, L. C. |
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Afiliação: |
KENNYA BEATRIZ SIQUEIRA, CNPGL; LUCAS CAMPIO PINHA, UFJF. |
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Título: |
Vantagens comparativas reveladas e o contexto do Brasil no comércio internacional de lacteos. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Informações Econômicas, v. 42, n. 3, p. 40-49, 2012 |
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Idioma: |
Português |
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Palavras-Chave: |
Leite e derivados; Setor lacteo. |
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Thesagro: |
Exportação. |
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Categoria do assunto: |
E Economia e Indústria Agrícola |
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Marc: |
LEADER 00501naa a2200157 a 4500
001 1943239
005 2024-02-05
008 2012 bl uuuu u00u1 u #d
100 1 $aSIQUEIRA, K. B.
245 $aVantagens comparativas reveladas e o contexto do Brasil no comércio internacional de lacteos.$h[electronic resource]
260 $c2012
650 $aExportação
653 $aLeite e derivados
653 $aSetor lacteo
700 1 $aPINHA, L. C.
773 $tInformações Econômicas$gv. 42, n. 3, p. 40-49, 2012
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Embrapa Gado de Leite (CNPGL) |
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